If you rely solely on hammer strike to obtain the desired fps in a regulated setup, then you risk being tuned far enough away from the plateau ( peak potential velocity with the given combo of barrel length/pressure/port size/pellet weight) that you will see a higher extreme spread than if you were tuned within 3-5% of your plateau. Reason being, is variation in hammer strikes (a hammer strike is rarely 100% consistent with its transfer of kinetic energy and momentum, temp / humidity swings, shot angle, and RNG all come into play here), as well as variation in pressure holding the valve closed (a regulator is rarely 100% consistent when it comes to packing just enough air molecules prior to closing itself off from the high pressure air contained within a reservoir .)
Really the 'best' tune takes into account all variables, the pressure (relative to the desired power output so one can be within 3-5% of their plateau), port size (relative to the power one desires), and hammer strike (relative to the needed lift/dwell to obtain desired power)
Harmonics is a pretty subjective subject...you smack a valve with a sprung weight to release a certain amount of air, there is no way to 'harmonically' tune that aspect...getting 900 fps at 1800 psi is relatively equal to getting 900 fps at 2500 psi provided you port each one so their plateaus are equal...since both setups would have the pellet arriving at the muzzle at 900 fps, what exactly is harmonic tuning in an airgun? BEATS ME, but people swear by it.
The only way to 'vary' harmonic tuning is respective to the pellet position in the barrel relative to barrel movement, which means varying its speed. So in the end, what ultimately is the observable difference if you were to 'harmonically tune' an airgun? The harmonics, or the slight variation / change in fps? With AIR rifles, operating at sub 5k pressure, there really isn't much, if any barrel movement. Barrel harmonics are well known in the PB world, but IMHO, 'harmonics' are irrelevant for us air gunners, just as much as spin stability is to a diablo pellet. Just my 2c and nothing more.
-Matt